|
KMID : 0616120060370040221
|
|
Korean Journal of Pharmacognosy
2006 Volume.37 No. 4 p.221 ~ p.228
|
|
|
Effect of Lectin Isolated from Serrognathus platymelus castanicolo Larvae on the Various Cytokine Expressions
|
|
Jo Su-Hyun
Kim Se-Jin
Chung See-Ryun
Chun Kyung-Hee
|
|
|
Abstract
|
|
|
|
A lectin was purified from Serrognathus platymelus castanicolo larvae and named as SPL. The purification was carried out by ion-exchange chromatography on DEAE Sephadex A-50 and gel filtration chromatography on Sephadex G-200. The purity of the protein was verified by polyacrylamide gel electrophoresis and the purified lectin agglutinated erythrocytes of rabbit and human A,B,O,AB. SPL was tested it’s ability to enhance the expressions of cytokines, IL-1α, IL-2, IL-6, TNFα and IFNγ by human peripheral blood mononuclear cells (PBMC) obtained from healthy donors. mRNA analyses were performed by RT-PCR at the moment of 1,4,8,24,48,72 and 96h after stimulation of PBMC with purified SPL. The patterns of IL-2 band were slightly expressed from 24h and the strongest band was appeared at 96th. The expression of Il-1α and IL-6 mRNA were strong from 1 to 8h and those of TNFα were from 48 to 96h. The mRNA encoding IFNγ were not detected. The addition of SPL for macrophage cultures induced production of nitric oxide(NO) by cells in a dose-dependent manner. NO release was partially inhibited by TNFα antibodies. These results suggest that SPL has the ability to enhance cytokine expressions in PBMC and to induce the NO release by TNFα in macrophage cultures from PBMC cultures.
|
|
|
KEYWORD
|
|
|
lectin, Serrognathus platymelus castanicolor, cytokine, immunomodulator, nitric oxide
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|